Biology Quiz and Test Materials for High School
High school biology helps students understand key concepts like cell structure and function, photosynthesis, genetics, evolution, ecosystems, and human impacts on the environment. These topics build a foundation for scientific thinking and prepare students for further study in life sciences.
Here you’ll find a variety of free biology quizzes and tests designed for grades 9–12. Teachers can use these materials for classroom assessments, homework, or practice. All quizzes and tests are ready to assign online or print, and you can copy and adapt them to fit your lesson plans.
By material type
Materials (18 materials)
Test
Protein synthesis test
This 10th-grade protein synthesis test helps students review key concepts such as transcription, translation, codons, anticodons, ribosomes, and mutations. Students practice multiple-choice and short-answer questions that assess understanding of each process and their roles in protein production.
Biology · Grade 10 · New · Oct 15, 2026
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DNA structure quiz
This 9th-grade quiz helps students practice identifying nucleotide components, base pairing rules, differences between DNA and RNA, and DNA replication enzymes through multiple-choice and select-all-that-apply questions, reinforcing key molecular biology concepts.
Biology · Grade 9 · New · Oct 15, 2026
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Pollution quiz
This 9th-grade pollution quiz helps students practice identifying sources and effects of pollutants, including acid rain, eutrophication, and plastic pollution, through multiple-choice and select-all questions that encourage critical thinking about environmental issues.
Biology · Grade 9 · New · Oct 15, 2026
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Human impacts on the environment test
This 10th-grade test challenges students to identify nonrenewable resources, major water pollutants, the effects of deforestation on biodiversity, and key greenhouse gases, using multiple-choice and short-answer questions to assess understanding of human impacts on the environment.
Biology · Grade 10 · New · Oct 15, 2026
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Cellular respiration test
This 10th-grade cellular respiration test helps students practice identifying stages, their cellular locations, ATP production levels, differences between aerobic and anaerobic pathways, and types of fermentation. The assessment includes multiple-choice questions on key processes and concepts.
Biology · Grade 10 · New · Oct 15, 2026
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Photosynthesis quiz
This 9th-grade photosynthesis quiz helps students practice identifying the overall equation, key reactants, and the role of chlorophyll through multiple-choice and select-all-that-apply questions. It also includes scenario-based questions on factors affecting the rate of photosynthesis.
Biology · Grade 9 · New · Oct 15, 2026
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Cell structure and transport test
This practice test helps 10th-grade students review cell theory, organelles, and types of transport through multiple-choice and short-answer questions. It encourages applying knowledge about cell structures and functions to different contexts.
Biology · Grade 10 · New · Oct 15, 2026
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Cell organelles quiz
This 9th-grade quiz helps students practice identifying cell organelles, their structures, and functions through multiple-choice and analogy questions. It encourages understanding of differences between plant and animal cells and the roles of key organelles.
Biology · Grade 9 · New · Oct 15, 2026
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Evolution test
This 10th-grade evolution test helps students practice identifying mechanisms of evolution, such as natural selection, genetic drift, gene flow, and mutation, through multiple-choice and short-answer questions. It also examines understanding of evidence for evolution and the process of speciation.
Biology · Grade 10 · New · Oct 15, 2026
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Natural selection quiz
This 9th-grade natural selection quiz helps students practice understanding Darwin's observations, adaptation, and fitness through multiple-choice and short-answer questions. It encourages critical thinking about natural selection processes and real-world examples.
Biology · Grade 9 · New · Oct 15, 2026
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Biodiversity quiz
This 9th-grade biodiversity quiz helps students practice understanding key concepts such as levels of biodiversity, keystone species, hotspots, causes of biodiversity loss, and conservation strategies. It includes multiple-choice questions and prompts to identify and explain these concepts.
Biology · Grade 9 · New · Oct 15, 2026
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Ecosystems and populations test
This test assesses 10th grade students' understanding of ecosystems and populations by including multiple-choice and short-answer questions on food webs, energy pyramids, population growth curves, and community interactions such as predation, competition, and symbiosis. It encourages application of concepts through interpretive questions.
Biology · Grade 10 · New · Oct 15, 2026
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Patterns of inheritance test
This 10th-grade worksheet helps students practice understanding inheritance patterns by answering multiple-choice and short-answer questions on Mendel's laws, incomplete and codominance, sex-linked traits, and pedigrees, enhancing their grasp of genetics concepts.
Biology · Grade 10 · New · Oct 15, 2026
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Punnett squares and genetics quiz
This quiz helps 9th-grade students practice genetics vocabulary, set up Punnett squares, and calculate probabilities of offspring traits through multiple-choice and short-answer questions. It reinforces understanding of genotype, phenotype, and inheritance patterns.
Biology · Grade 9 · New · Oct 15, 2026
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Body systems test
An 11th-grade test on body systems that helps students practice identifying major organs and their functions, as well as understanding how different systems interact to maintain homeostasis. The questions include multiple-choice and short-answer formats.
Biology · Grade 11 · New · Oct 15, 2026
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Homeostasis quiz
This 10th-grade homeostasis quiz helps students practice understanding key concepts like set points, negative feedback mechanisms, thermoregulation, water balance, and kidney function through multiple-choice questions. It encourages critical thinking about how the body maintains stability and what happens when homeostasis fails.
Biology · Grade 10 · New · Oct 15, 2026
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Mitosis and meiosis test
This 10th-grade test helps students practice identifying phases of mitosis and meiosis through multiple-choice and select-all questions, focusing on chromosome behavior, homologous versus sister chromatids, and the purpose of each division.
Biology · Grade 10 · New · Oct 15, 2026
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Cell cycle and mitosis quiz
This 9th-grade quiz helps students practice identifying stages of the cell cycle, including interphase, mitosis, checkpoints, and the connection to cancer. It features multiple-choice and select-all questions to reinforce understanding of cell division processes.
Biology · Grade 9 · New · Oct 15, 2026
Topics
- Punnett Square Worksheets for Inheritance and Variation (Grades 9–12)
- Protein Synthesis Worksheets for High School Biology
- Photosynthesis Worksheets for High School Biology
- Mitosis Worksheets and Meiosis Resources for High School Biology
- Body Systems Worksheets: Homeostasis and Body Systems (Grades 9–12)
- Cell Structure and Function Worksheets for High School Biology
- Evolution Worksheets for High School Biology: Natural Selection
- Biodiversity Worksheets for High School Ecosystems and Populations
- Pollution Worksheets for Human Impacts on the Environment (Grades 9–12)
Questions teachers ask
What topics do the biology quizzes cover?
The quizzes cover high school biology topics such as cell structure, photosynthesis, genetics, evolution, ecosystems, and human impacts on the environment.
Can I edit the biology test materials?
Yes, you can copy any quiz or test from the library and edit the questions or format to suit your class needs.
How can I use these quizzes with my students?
You can assign quizzes online with a class code or print them for in-class or homework use. Automatic grading is available for online assignments.