SLM Biotech Q1 M6 (Output)

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What I Know Directions: Choose the letter of the correct answer. Write your answers in your notebook. 1. What biological technique is used to study the internal organs of specific organism? A. dissection C. microscopy B. tissue culture D. aseptic technique 2. Aseptic technique is synonymous with clean technique. A. True C. Maybe B. False D. Cannot be determined 3. Which of the following basic microscopy is appropriate to use in viewing bacteria or detail of fixed specimens? A. dark-field microscopy C. oil immersion microscopy B. bright-field microscopy D. phase contrast microscopy 4. Blunt dissection can be done by . A. fingers C. Both A and B B. probe D. None of the choices 5. Is it possible to have increased magnification without increased resolution? A. Yes C. Maybe B. No D. Cannot be determined 6. It is a set of infection control practices that it is necessary to use in operating rooms. A. Clean technique C. Aseptic technique B. Sterile technique D. Biological technique 7. _____ tissue culture is a type of tissue culture that involves isolating an organ for in vitro growth. A. Organ C. Embryo B. Callus D. Seed 8. It is carried out for making museum specimens. It appreciates the depth and relations of the deeper structures. What type of dissection is described? A. uni-structural C. comprehensive study B. Composite Window Corridor D. composite study 9. For plants undergoing tissue culture it needs . A. higher concentration of calcium C. normal concentration of calcium B. lower concentration of calcium D. cannot be determined 10. Aseptic technique is not the same as surgical asepsis. A. Yes C. Maybe B. No D. Cannot be determined 1

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What’s New Activity No. 2: Experiment: The True Colors of Leaves! Objective: Identify the biological techniques, procedures and methods. Directions: Prepare the materials needed materials for the simple experiment below. Perform them and answer the given guide questions. What is the color of the leaves? Perhaps, your answer would be green, because But, is it the only pigment that leaves have? Do this activity to see if there are hidden colors in green leaves! Note: Adult supervision is recommended. Materials: • green leaves • tissue • glass/ plastic cup/wide-mouthed jar • pencil • mortar and pestle (almires or dikdikan) • dish or small basin • isopropyl rubbing alcohol • hot water • used cardboard or folder Procedure: 1. Tear the leaves into several pieces. If you have mortar and pestle you can use it to pound and extract the green juice from the leaves then put it in a glass. Add just enough rubbing alcohol to cover them. Put a cardboard on top of the glass to keep the alcohol from evaporating. 2. Put the glass in a dish with hot tap water ask for adult assistance in doing this step) for about 30 minutes, until the alcohol turns green. 3. Cut a strip of tissue paper about a half inch wide and tape it to a pencil. Suspend the pencil across the glass and let the strip just barely touch the alcohol and pigment mixture. Leave for about 30-60 minutes. Caution: the solvent (alcohol) is highly flammable and gives off potentially harmful fumes. Do not directly smell the solution. Source: https://learning-center.homesciencetools.com/article/leaf-chromatography- science-project/ 2

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Guide Questions: 1. What colors become visible in your chromatography paper or tissue? 2. Which of the colors travelled highest in the paper/tissue? Which travelled the lowest? 2. Were you able to separate the colors or pigments of the green mixture? 4. What do you call the process of separating the mixtures of pigments in the leaves? 3

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What’s More Activity No. 3: Graphic Organizer: Biological Techniques Objective: Describe each biological technique, procedures and method. Directions: Complete the table below by writing a brief description of Biological Techniques in column 2 and write one uses or application of each technique in column 3. Refer your answer on the attached hand-out. BIOLOGICAL APPLICATION/ DESCRIPTION TECHNIQUE USES 1. Cloning 2. DNA Sequencing 3. Cell and Tissue Culture 4. Centrifugation 5. Chromatography 6. Gel Electrophoresis 7. Spectrophotometry 8. Polymerase Chain Reaction 4

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What I Have Learned Activity No. 4: Short Essay: Importance of Biological Techniques Objective: Appreciate the importance of biological techniques to human life. Directions: Explain the importance of biological techniques to human life specifically in the production of vaccines and medicines during the Covid-19 pandemic. 5

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Assessment Directions: Choose the letter of the correct answer. Write your answers in your notebook. 1. What biological technique should be applied to prevent contamination from pathogens? A. dissection C. microscopy B. tissue culture D. aseptic technique 2. Aseptic technique is synonymous with sterile technique. A. True C. Maybe B. False D. Cannot be determined 3. Which of the following basic microscopy is appropriate to use for a transparent and high magnifications (400x, 1000x)? A. dark-field microscopy C. oil immersion microscopy B. bright-field microscopy D. phase contrast microscopy 4. Sharp dissection can be done by . A. fingers C. Both A and B B. probe D. None of the choices 5. Is it possible to have increased magnification and no change in resolution? A. Yes C. Maybe B. No D. Cannot be determined 6. It is a method or procedure that is used to study living things. A. Clean technique C. Aseptic technique B. Sterile technique D. Biological technique 7. _____ tissue culture is often sustained on a gel medium, which is composed of agar and a mixture of given macro and micronutrients depending on the type of cells. A. Organ C. Embryo B. Callus D. Seed 8. It is carried out for demonstration and display purpose. In this study dissection is carried out in a particular region or a part of body where some parts may be removed totally or partly. What type of dissection is described? A. uni-structural C. comprehensive study B. Composite Window Corridor D. composite study 9. For plants undergoing tissue culture it needs lower concentration of calcium. A. True C. Maybe B. False D. Cannot be determined 10. Aseptic technique is the same as surgical asepsis. A. Yes C. Maybe B. No D. Cannot be determined 6